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Image Search Results
Journal: Cell Death & Disease
Article Title: Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis
doi: 10.1038/s41419-021-03755-z
Figure Lengend Snippet: A Immunofluorescence staining of SYCP3 (green) and γH2AX (red) in sections of testes from the WT and Cep70 −/− mice. DAPI indicates the nucleus. Scale bar: 100 μm (up), 50 μm (down). B Immunofluorescence staining of SYCP3 (green) and γH2AX (red) in chromosome spreads of spermatocytes from the testes of 10-week-old WT and Cep70 −/− male mice. Scale bar: 5 μm. C Quantification of the proportion of meiotic stages in WT and Cep70 −/− spermatocytes. The numbers marked in the bars represent the percentage of cells at indicated meiosis stage (leptotene, zygotene, pachytene, and diplotene). For each genotype, three mice were analyzed. p -values were calculated by Student’s t -test. Data are presented as the mean ± SD, n.s. not statistically significant.
Article Snippet: Then, the tissue sections were blocked with 3% bovine serum albumin (BSA) for 1 h, and incubated overnight at 4 °C with the following antibodies: anti-MVH (Abcam, ab13840, 1:500, rabbit), anti-SYCP3 (Abcam, ab97672, 1:100, mouse),
Techniques: Immunofluorescence, Staining
Journal: PLoS ONE
Article Title: A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
doi: 10.1371/journal.pone.0154610
Figure Lengend Snippet: (A) Bar graph of the measurement of Ki67 positive cells, showing the fraction of cycling Calu-3 cells after static and dynamic suspension culture (*: p<0.05 vs static suspension). (B) Bar graph of the measurement of γH2AX positive cells, quantifying the double DNA strand breaks in Calu-3 cells harvested from static and dynamic suspension culture.
Article Snippet: Cell spots were stained by anti-Ki67 (Ki67, mouse monoclonal, DAKO, Italy) and
Techniques: Suspension